Hornerin Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-93405
Key Product Details
Species Reactivity
Human
Applications
Peptide ELISA
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-QYATQHGEYDTLNK corresponding to internal region according to NP_001009931.1.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Hornerin Antibody
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Hornerin Antibody [NB100-93405] -
Immunocytochemistry/Immunofluorescence: Goat Polyclonal Hornerin Antibody [NB100-93405] - Immunofluorescence analysis of paraformaldehyde fixed U251 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
Flow Cytometry: Goat Polyclonal Hornerin Antibody [NB100-93405] -
Flow Cytometry: Goat Polyclonal Hornerin Antibody [NB100-93405] - Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.Applications for Hornerin Antibody
Application
Recommended Usage
Peptide ELISA
Detection limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 50 kDa band in human skin lysates after 0.3 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 282 kDa band according to NP_001009931.1. The 50 kDa band was successfully blocked by incubation with the immunizing peptide.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Hornerin
Alternate Names
hornerin, intermediate filament-associated protein, S100A16, S100A18
Entrez Gene IDs
388697 (Human)
Gene Symbol
HRNR
UniProt
Additional Hornerin Products
Product Documents for Hornerin Antibody
Product Specific Notices for Hornerin Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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