Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Chemotaxis, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all HP1 alpha Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468).

Marker

Heterochromatin Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HP1 alpha Antibody - BSA Free

Western Blot: HP1 alpha Antibody [NB110-40623]

Western Blot: HP1 alpha Antibody [NB110-40623]

Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]

Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]

Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB

Applications for HP1 alpha Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

1:10-1:500

Immunohistochemistry

1:200-1:10000

Immunohistochemistry-Paraffin

1:200-1:10000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:1000-1:10000
Application Notes
Chip, IP reactivity reported in (PMID: 23045645). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HP1 alpha

CBX5 (chromobox protein homolog 5), also known as HP1 (heterochromatin protein 1 homolog alpha), is one of 3 isoforms of HP1 that binds heterochromatin and functions as a structural adaptor to stabilize and organize macromolecular complexes with nucleosomes for higher chromatin structure. All of the HP1 isoforms possess a chromodomain and chromoshadow domain and associate with methylated histones that mediate transcriptional repression. Alternate names for CBX5 include HP1 alpha, antigen p25, HP1Hs-alpha, and HP1A.

Alternate Names

Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha

Entrez Gene IDs

23468 (Human)

Gene Symbol

CBX5

UniProt

P45973 (Human)

Additional HP1 alpha Products

Product Documents for HP1 alpha Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HP1 alpha Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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