HPF1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-93973
![Simple Western: HPF1 Antibody [NBP1-93973]](https://resources.bio-techne.com/images/products/HPF1-Antibody-Simple-Western-NBP1-93973-img0007.jpg "Simple Western: HPF1 Antibody [NBP1-93973]")
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Rat
Predicted:
Mouse (92%), Rat (91%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LVGPYDILAGKHKTKKKSTGLNFNLHWRFYYDPPEFQTIIIGDNKTQYHMGYFRDSPDEFPVYVGINEAKKNCIIVPNGDNVFAA
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HPF1 Antibody
![Western Blot: HPF1 Antibody [NBP1-93973]](https://resources.bio-techne.com/images/products/HPF1-Antibody-Western-Blot-NBP1-93973-img0013.jpg "Western Blot: HPF1 Antibody [NBP1-93973]")
Western Blot: HPF1 Antibody [NBP1-93973]
Western Blot: HPF1 Antibody [NBP1-93973] - Analysis in human cell line MOLT-4.![Immunocytochemistry/ Immunofluorescence: HPF1 Antibody [NBP1-93973]](https://resources.bio-techne.com/images/products/HPF1-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-93973-img0012.jpg "Immunocytochemistry/ Immunofluorescence: HPF1 Antibody [NBP1-93973]")
Immunocytochemistry/ Immunofluorescence: HPF1 Antibody [NBP1-93973]
Immunocytochemistry/Immunofluorescence: HPF1 Antibody [NBP1-93973] - Staining of human cell line U-251 shows localization to nucleoli, nucleoplasm and cytosol. Antibody staining is shown in green.![Simple Western: HPF1 Antibody [NBP1-93973]](https://resources.bio-techne.com/images/products/HPF1-Antibody-Simple-Western-NBP1-93973-img0006.jpg "Simple Western: HPF1 Antibody [NBP1-93973]")
Simple Western: HPF1 Antibody [NBP1-93973]
Simple Western: HPF1 Antibody [NBP1-93973] - Simple Western lane view shows a specific band for C4orf27 in 0.2 mg/ml of MOLT-4 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.Applications for HPF1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Simple Western
1:25
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HPF1
Alternate Names
chromosome 4 open reading frame 27, FLJ20534, FLJ33423
Gene Symbol
C4orf27
Additional HPF1 Products
Product Documents for HPF1 Antibody
Product Specific Notices for HPF1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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