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HPRT Antibody (4C3-G8)

Novus Biologicals, part of Bio-Techne | Catalog # H00003251-M01

Novus Biologicals, part of Bio-Techne
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H00003251-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG Clone # 4C3-G8

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HPRT1 (AAH00578, 1 a.a. ~ 218 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MATRSPGVVISDDEPGYDLDLFCIPNHYAEDLERVFIPHGLIMDRTERLARDVMKEMGGHHIVALCVLKGGYKFFADLLDYIKALNRNSDRSIPMTVDFIRLKSYCNDQSTGDIKVIGGDDLSTLTGKNVLIVEDIIDTGKTMQTLLSLVRQYNPKMVKVASLLVKRTPRSVGYKPDFVGFEIPDKFVVGYALDYNEYFRDLNHVCVISETGKAKYKA

Specificity

HPRT1 - hypoxanthine phosphoribosyltransferase 1 (Lesch-Nyhan syndrome)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HPRT Antibody (4C3-G8)

Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01]

Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01]

Western Blot: HPRT Antibody (4C3-G8) [H00003251-M01] - HPRT1 monoclonal antibody (M01), clone 4C3-G8 Analysis of HPRT1 expression in MCF-7.

Applications for HPRT Antibody (4C3-G8)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HPRT

HPRT1 has a central role in the generation of purine nucleotides through the purine salvage pathway. HPRT1 catalyzes conversion of hypoxanthine to inosine monophosphate and guanine to guanosine monophosphate via transfer of the 5-phosphoribosyl group from 5-phosphoribosyl 1-pyrophosphate (Keebaugh et al., 2007 [PubMed 16928426]).[supplied by OMIM]

Long Name

Hypoxanthine-Guanine Phosphoribosyltransferase

Alternate Names

HGPRT, HGPRT1, HPRT1

Entrez Gene IDs

3251 (Human)

Gene Symbol

HPRT1

OMIM

300 (Human)

Additional HPRT Products

Product Documents for HPRT Antibody (4C3-G8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HPRT Antibody (4C3-G8)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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