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HSD17B3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16884

Novus Biologicals, part of Bio-Techne
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NBP2-16884

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human HSD17B3. The exact sequence is proprietary.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSD17B3 Antibody

Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-16884]

Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-16884]

Immunohistochemistry-Paraffin: HSD17B3 Antibody [NBP2-16884] - Human hepatoma, using HSD17B3 antibody antibody at 1:500 dilution.Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Immunocytochemistry/ Immunofluorescence: HSD17B3 Antibody [NBP2-16884]

Immunocytochemistry/ Immunofluorescence: HSD17B3 Antibody [NBP2-16884]

Immunocytochemistry/Immunofluorescence: HSD17B3 Antibody [NBP2-16884] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: HSD17B3 protein stained by HSD17B3 antibody diluted at 1:500. Blue: Hoechst 33342 staining.
HSD17B3 Antibody

Western Blot: HSD17B3 Antibody [NBP2-16884] -

Western Blot: HSD17B3 Antibody [NBP2-16884] - Various whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSD17B3 antibody (NBP2-16884) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for HSD17B3 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSD17B3

This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq]

Alternate Names

EC 1.1.1.64, EDH17B317-beta-HSD3, hydroxysteroid (17-beta) dehydrogenase 3,17-beta-hydroxysteroid dehydrogenase type 3, SDR12C2, short chain dehydrogenase/reductase family 12C, member 2, Testicular 17-beta-hydroxysteroid dehydrogenase, testosterone 17-beta-dehydrogenase 3,17-beta-HSD 3

Gene Symbol

HSD17B3

UniProt

Additional HSD17B3 Products

Product Documents for HSD17B3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSD17B3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.