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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI4C4

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human HSD17B4(NP_000405) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

Western Blot: HSD17B4 Antibody (OTI4C4)Azide and BSA Free [NBP2-70947]

Western Blot: HSD17B4 Antibody (OTI4C4)Azide and BSA Free [NBP2-70947]

Western Blot: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSD17B4.
Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]

Immunocytochemistry/ Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]

Immunocytochemistry/Immunofluorescence: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY HSD17B4.
Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947]

Immunohistochemistry: HSD17B4 Antibody (OTI4C4) - Azide and BSA Free [NBP2-70947] - Analysis of Human prostate tissue.

Applications for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Western Blot

1:4000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSD17B4

The protein encoded by the HSD17B4 gene is a bifunctional enzyme that is involved in the peroxisomal beta-oxidation pathwayfor fatty acids. It also acts as a catalyst for the formation of 3-ketoacyl-CoA intermediates from both straight-chainand 2-methyl-branched-chain fatty acids. Defects in this gene that affect the peroxisomal fatty acid beta-oxidationactivity are a cause of D-bifunctional protein deficiency (DBPD). An apparent pseudogene of this gene is present onchromosome 8. (provided by RefSeq)

Alternate Names

12-alpha-trihydroxy-5-beta-cholest-24-enoyl-CoA hydratase, 7-alpha, D-bifunctional protein, EDH17B4, hydroxysteroid (17-beta) dehydrogenase 4, MPF-2, peroxisomal, peroxisomal multifunctional enzyme type 2,17-beta-HSD IV, peroxisomal multifunctional protein 2,17beta-estradiol dehydrogenase type IV, short chain dehydrogenase/reductase family 8C, member 1,3-alpha

Gene Symbol

HSD17B4

Additional HSD17B4 Products

Product Documents for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSD17B4 Antibody (OTI4C4) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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