HSF1 Antibody (1A11)

Novus Biologicals, part of Bio-Techne | Catalog # H00003297-M09

Novus Biologicals, part of Bio-Techne
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H00003297-M09
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A11

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSF1 Antibodies »

Product Specifications

Immunogen

HSF1 (NP_005517.1, 256 a.a. ~ 359 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. DAVASSGPIISDITELAPASPMASPGGSIDERPLSSSPLVRVKEEPPSPPQSPRVEEASPGRPSSVDTLLSPTALIDSILRESEPAPASVTALTDARGHTDTEG

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HSF1 Antibody (1A11)

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (1A11) [H00003297-M09]

Immunocytochemistry/ Immunofluorescence: HSF1 Antibody (1A11) [H00003297-M09]

Immunocytochemistry/Immunofluorescence: HSF1 Antibody (1A11) [H00003297-M09] - Analysis of monoclonal antibody to HSF1 on HeLa cell. Antibody concentration 20 ug/ml
Sandwich ELISA: HSF1 Antibody (1A11) [H00003297-M09] - Detection limit for recombinant GST tagged HSF1 is 3 ng/ml as a capture antibody.

Applications for HSF1 Antibody (1A11)

Application
Recommended Usage

Sandwich ELISA

1:100-1:2000

Western Blot

1:100-1:2000
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

UniProt

NP_005517 (Human)

Additional HSF1 Products

Product Documents for HSF1 Antibody (1A11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSF1 Antibody (1A11)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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