HSF1 [p Ser326] Antibody (2A7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26417

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-26417-100ul
NBP3-26417-50ul
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2A7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSF1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human HSF1 [p Ser326] [UniProt Q00613]

Modification

p Ser 326

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSF1 [p Ser326] Antibody (2A7)

HSF1 [p Ser326] Antibody (2A7)

Western Blot: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] -

Western Blot: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] - Positive Western Blot detected in Hela whole cell lysate.
All lanes: HSF1 [p Ser326] Antibody at 0.8ug/ml.
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 82 KDa
Observed band size: 82 KDa
HSF1 [p Ser326] Antibody (2A7)

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] -

Immunocytochemistry/Immunofluorescence: HSF1 [p Ser326] Antibody (2A7) [NBP3-26417] - Analysis of HSF1 [p Ser326] Antibody (2A7) diluted at 1:80 and staining in Hela cells. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Applications for HSF1 [p Ser326] Antibody (2A7)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HSF1

Human cells respond to heat stress by inducing the binding of a pre-existing transcriptional activator (heat shock factor, HSF) to DNA (1). Induction of heat shock protein (HSP) gene expression by stress is initiated by binding of HSF1 to HSP gene promoters to increase their transcription. The cytoprotective functions of these HSPs are essential for cell survival, and thus it is critical that inducible HSP gene expression be executed rapidly and efficiently. There is an interaction between heat shock factor 1 (HSF1) and symplekin, a protein known to form a complex with the polyadenylation factors CstF and CPSF. HSF1-symplekin complexes are detected only after stress treatment, and these two proteins co-localize in punctate nuclear structures in stressed cells (2). A chaperone/Hsp functioning as repressor of heat shock transcription factor (HSF) could make activation of hsp genes dependent on protein unfolding. It has been concluded that Hsp90, by itself and/or associated with multichaperone complexes, is a major repressor of HSF1 (3).

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for HSF1 [p Ser326] Antibody (2A7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSF1 [p Ser326] Antibody (2A7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.