HSF1 [p Ser326] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97452

Novus Biologicals, part of Bio-Techne
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NBP1-97452-0.2mg
NBP1-97452-0.05mg
NBP1-97452-0.025mg
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Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSF1 Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to the N-terminus of human HSF1 phosphorylated at Ser 326.

Modification

p Ser326

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSF1 [p Ser326] Antibody

Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452]

Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452]

Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of Recombinant HSF1 and HSF1 (phospho): Lane 1: MWM, Lane 2: Recombinant HSF1, Lane 3: Recombinant HSF1 (phospho), probed with HSF1 (pSer326) pAb.
Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452]

Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452]

Western Blot: HSF1 [p Ser326] Antibody [NBP1-97452] - Analysis of HSF1 (pSer326): Lane 1: MW marker, Lane 2: HeLa, Lane 3: HeLa, Heat-Shocked (No recovery time), probed with HSF1 (pSer326) pAb.

Applications for HSF1 [p Ser326] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
Predicted MW of ~60kDa. Detects a band of 85-95kDa by Western blot depending on hyperphosphorylation of the native protein.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSF1

HSFs (Heat Shock family of transcription factors), which consists of HSF 1-4, bind to highly conserved Heat shock elements (HSEs) in the promoter regions of heat shock genes, ultimately regulating the expression of Heat shock proteins (Hsps). On exposure to heat shock and other stresses, HSF1 localizes within seconds to discrete nuclear granules and on recovery from stress, HSF1 rapidly dissipates from the stress granules to a diffuse nucleoplasmic distribution.

Long Name

Heat Shock Transcription Factor 1

Alternate Names

HSTF1

Gene Symbol

HSF1

Additional HSF1 Products

Product Documents for HSF1 [p Ser326] Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSF1 [p Ser326] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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