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HSF2 Antibody (1F11-A3)

Novus Biologicals, part of Bio-Techne | Catalog # H00003298-M01

Novus Biologicals, part of Bio-Techne
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H00003298-M01

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1F11-A3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

HSF2 (AAH05329, 1 a.a. ~ 230 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MKQSSNVPAFLSKLWTLVEETHTNEFITWSQNGQSFLVLDEQRFAKEILPKYFKHNNMASFVRQLNMYGFRKVVHIDSGIVKQERDGPVEFQHPYFKQGQDDLLENIKRKVSSSKPEENKIRQEDLTKIISSAQKVQIKQETIESRLSELKSENESLWKEVSELRAKHAQQQQVIRKIVQFIVTLVQNNQLVSLKRKRPLLLNTNGAQKKNLFQHIVKEPTDNHHHKVIF

Localization

Cytoplasmic during normal growth and moves to the nucleus upon activation.

Specificity

HSF2 - heat shock transcription factor 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for HSF2 Antibody (1F11-A3)

Immunocytochemistry/ Immunofluorescence: HSF2 Antibody (1F11-A3) [H00003298-M01]

Immunocytochemistry/ Immunofluorescence: HSF2 Antibody (1F11-A3) [H00003298-M01]

Immunocytochemistry/Immunofluorescence: HSF2 Antibody (1F11-A3) [H00003298-M01] - Analysis of monoclonal antibody to HSF2 on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: HSF2 Antibody (1F11-A3) [H00003298-M01] - Detection limit for recombinant GST tagged HSF2 is approximately 0.3ng/ml as a capture antibody.

Applications for HSF2 Antibody (1F11-A3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSF2

HSF2, as well as the related gene HSF1, encodes a protein that binds specifically to the heat-shock element and has homology to HSFs of other species. Heat shock transcription factors activate heat-shock response genes under conditions of heat or other stresses. Although the names HSF1 and HSF2 were chosen for historical reasons, these peptides should be referred to as heat-shock transcription factors.

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Entrez Gene IDs

3298 (Human)

Gene Symbol

HSF2

OMIM

140581 (Human)

Additional HSF2 Products

Product Documents for HSF2 Antibody (1F11-A3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSF2 Antibody (1F11-A3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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