Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Guinea Pig, Hamster, Primate, Rabbit, Sheep

Applications

Immunocytochemistry/ Immunofluorescence, Microarray, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 3E2

Concentration

1.0 mg/ml

View all HSF2 Antibodies »

Product Specifications

Immunogen

Purified recombinant mouse HSF2 protein

Reactivity Notes

Human, Mouse, Rat, Guinea Pig, Hamster, Primate, Rabbit, Bovine, Sheep, Porcine, Canine

Localization

Cytoplasm , Nucleus

Specificity

Detects an approx. 69kDa protein in unstressed cells corresponding to HSF2 on SDS-PAGE immunoblots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for HSF2 Antibody (3E2)

Western Blot: HSF2 Antibody (3E2) [NB110-96434]

Western Blot: HSF2 Antibody (3E2) [NB110-96434]

Western Blot: HSF2 Antibody (3E2) [NB110-96434] - Analysis (K562 cells) transiently transfected with control, HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland.

Applications for HSF2 Antibody (3E2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:200

Western Blot

4 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSF2

HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5). Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6). There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

Long Name

Heat Shock Transcription Factor 2

Alternate Names

HSTF2

Entrez Gene IDs

3298 (Human); 15500 (Mouse); 64441 (Rat)

Gene Symbol

HSF2

UniProt

Q03933

Additional HSF2 Products

Product Documents for HSF2 Antibody (3E2)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSF2 Antibody (3E2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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