HSP20/HSPB6 Antibody (6A4)

Novus Biologicals, part of Bio-Techne | Catalog # H00126393-M03

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Lambda Clone # 6A4

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP20/HSPB6 Antibodies »

Product Specifications

Immunogen

HSPB6 (AAH68046.1, 1 a.a. ~ 160 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MEIPVPVQPSWLRRASAPLLGLSAPGRLFDQRFGEGLLEAELAALCPTTLAPYYLRAPSVALPVAQVPTDPGHFSVLLDVKHFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGVLSIQAAPASAQAPPPAAAK

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Lambda

Scientific Data Images for HSP20/HSPB6 Antibody (6A4)

Western Blot: HSP20/HSPB6 Antibody (6A4) [H00126393-M03]

Western Blot: HSP20/HSPB6 Antibody (6A4) [H00126393-M03]

Western Blot: HSP20/HSPB6 Antibody (6A4) [H00126393-M03] - Analysis of HSPB6 expression in transfected 293T cell line by HSPB6 monoclonal antibody (M03), clone 6A4. Lane 1: HSPB6 transfected lysatE (17.2 KDa). Lane 2: Non-transfected lysate.
Immunocytochemistry/ Immunofluorescence: HSP20/HSPB6 Antibody (6A4) [H00126393-M03]

Immunocytochemistry/ Immunofluorescence: HSP20/HSPB6 Antibody (6A4) [H00126393-M03]

Immunocytochemistry/Immunofluorescence: HSP20/HSPB6 Antibody (6A4) [H00126393-M03] - Analysis of monoclonal antibody to HSPB6 on HeLa cell. Antibody concentration 10 ug/ml
Sandwich ELISA: HSP20/HSPB6 Antibody (6A4) [H00126393-M03] - Detection limit for recombinant GST tagged HSPB6 is 0.3 ng/ml as a capture antibody.

Applications for HSP20/HSPB6 Antibody (6A4)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This antibody is useful for ELISA, Western Blot

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSP20/HSPB6

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Gene Symbol

HSPB6

UniProt

AAH68046 (Human)

Additional HSP20/HSPB6 Products

Product Documents for HSP20/HSPB6 Antibody (6A4)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP20/HSPB6 Antibody (6A4)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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