HSP20/HSPB6 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB120-13491

Novus Biologicals, part of Bio-Techne
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NB120-13491
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Fish

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all HSP20/HSPB6 Antibodies »

Product Specifications

Immunogen

Rat skeletal muscle Hsp20.

Specificity

Hsp20

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP20/HSPB6 Antibody

Western Blot: HSP20/HSPB6 Antibody [NB120-13491]

Western Blot: HSP20/HSPB6 Antibody [NB120-13491]

Western Blot: HSP20/HSPB6 Antibody [NB120-13491] - Lane 1: MW Marker, Lane 2: HeLa (heat shocked), Lane 3: 3T3 (heat shocked), Lane 4: PC-12 (heat shocked).

Applications for HSP20/HSPB6 Antibody

Application
Recommended Usage

Western Blot

1:1000 (colorimetric)
Application Notes
Detects a band of ~20 kDa by Western blot.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

0.1M Phosphate (pH 7.0) and 3% BSA

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP20/HSPB6

Hsp20 is a small heat shock protein related to Hsp25, Hsp27 and may form different hetercomplexes with these proteins. The specific physiological function of Hsp20 is not yet known. It is distributed ubiquitously in tissues, but is found in higher levels in skeletal, smooth and heart muscle. Under normal conditions, Hsp20 is diffusely distributed in the cytosol, but under heat stress conditions, it translocates to the nucleus. Unlike other heat shock proteins the amount of Hsp20 does not increase after heat shock. The Hsp20 was demonstrated to constitute up to 1.3% of the total cellular protein in vertebrate tissues, especially in muscle, and its expression is related to muscle contraction, specifically in slow-twitch muscle. Hsp20 may form different heterocomplexes with other Hsp's, such as alpha-crystalline and Hsp25. Phosphorylated form of Hsp20 is proposed to interact with monomeric actin whereas dephosphorylated form binds polymeric actin filaments. In normal conditions Hsp20 is diffusely disturbed in cytosol but under the heat stress it undergoes translocation to membrane fraction.

Long Name

Heat Shock Protein 20

Alternate Names

HSPB6

Entrez Gene IDs

126393 (Human); 243912 (Mouse); 192245 (Rat)

Gene Symbol

HSPB6

UniProt

P97541

Additional HSP20/HSPB6 Products

Product Documents for HSP20/HSPB6 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP20/HSPB6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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