HSP27 [p Ser82] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-13297

Novus Biologicals, part of Bio-Techne
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NBP3-13297
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Canine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser82 of human HSP27. The exact sequence is proprietary.

Modification

p Ser 82

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for HSP27 [p Ser82] Antibody

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) C2C12 whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297]

Western Blot: HSP27 [p Ser82] Antibody [NBP3-13297] - Whole cell extract (30 ug) was separated by 12% SDS-PAGE, and the membrane was blotted with HSP27 (phospho Ser82) antibody (NBP3-13297) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Applications for HSP27 [p Ser82] Antibody

Application
Recommended Usage

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS (pH 7), 20% Glycerol, 1% BSA

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 [p Ser82] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 [p Ser82] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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