Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1C4-1A6

Concentration

1 mg/ml

View all HSP47 Antibodies »

Product Summary for Hsp47 Antibody (1C4-1A6)

Immunogen

Human HSP47, full length

Localization

Endoplasmic Reticulum , Endoplasmic Reticulum Lumen

Specificity

Detects 47kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for Hsp47 Antibody (1C4-1A6)

Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Western Blot: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - analysis of Human Epithelial cell (A431) lysates showing detection of 47 kDa Hsp47 protein using Mouse Anti-Hsp47 Monoclonal Antibody, Clone 1C4-1A6 . Lane 1: MW ladder. Lane 2: Anti-Hsp47 (1:250). Lane 3: Anti-Hsp47 (1:500). Lane 4: Anti-Hsp47 (1:1000). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:250 - 1:1000 for 1 hour at RT. Secondary Antibody: HRP Goat Anti-Mouse at 1:50 for 1 hour at RT. Color Development: TMB solution for 10 min at RT. Predicted/Observed Size: 47 kDa.
Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 100x.
Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400]

Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (1C4-1A6) [NBP2-22400] - Tissue: Heat Shocked HeLa Cells. Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-Hsp47 Monoclonal Antibody at 1:100 for 12 hours at 4C. Secondary Antibody: APC Goat Anti-Mouse (red) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Localization: Endoplasmic reticulum lumen. Cytoplasm. Magnification: 20x.

Applications for Hsp47 Antibody (1C4-1A6)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Application Notes
1 ug/ml of HSP47 Antibody was sufficient for detection of HSP47 in 20 ug of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary Antibody.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP47

Hsp47 encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Gene Symbol

SERPINH1

UniProt

P50454.2 (Human)

Additional HSP47 Products

Product Documents for Hsp47 Antibody (1C4-1A6)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Hsp47 Antibody (1C4-1A6)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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