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Hsp47 Antibody (1D2-1A6)

Novus Biologicals, part of Bio-Techne | Catalog # H00000871-M01

Novus Biologicals, part of Bio-Techne
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H00000871-M01

Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D2-1A6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

SERPINH1 (AAH14623.1, 1 a.a. ~ 418 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

Specificity

SERPINH1 - serine (or cysteine) proteinase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Hsp47 Antibody (1D2-1A6)

Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2. Analysis of SERPINH1 expression in rat brain.
Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Immunohistochemistry-Paraffin: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - Immunoperoxidase of monoclonal antibody to SERPINH1 on formalin-fixed paraffin-embedded human ovary cancer. [antibody concentration 5 ug/ml]
Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01]

Western Blot: Hsp47 Antibody (1D2-1A6) [H00000871-M01] - SERPINH1 monoclonal antibody (M01), clone S2 Analysis of SERPINH1 expression in HeLa.

Applications for Hsp47 Antibody (1D2-1A6)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: HSP47

This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. Its expression is induced by heat shock. The protein localizes to the endoplasmic reticulum lumen and binds collagen; thus it is thought to be a molecular chaperone involved in the maturation of collagen molecules. Autoantibodies to this protein have been found in patients with rheumatoid arthritis.

Long Name

Heat Shock Protein 47

Alternate Names

AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1

Entrez Gene IDs

871 (Human)

Gene Symbol

SERPINH1

OMIM

600943 (Human)

Additional HSP47 Products

Product Documents for Hsp47 Antibody (1D2-1A6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Hsp47 Antibody (1D2-1A6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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