Hsp47 Antibody (3K3H1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15430
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3K3H1
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for Hsp47 Antibody (3K3H1)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 319-418 of human Hsp47 (P50454). KHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hsp47 Antibody (3K3H1)
![Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430]](https://resources.bio-techne.com/images/products/Hsp47-Antibody-3K3H1-Western-Blot-NBP3-15430-img0003.jpg "Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430]")
Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430]
Western Blot: Hsp47 Antibody (3K3H1) [NBP3-15430] - Western blot analysis of extracts of various cell lines, using Hsp47 Rabbit mAb (NBP3-15430) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.![Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430]](https://resources.bio-techne.com/images/products/Hsp47-Antibody-3K3H1-Immunocytochemistry-Immunofluorescence-NBP3-15430-img0002.jpg "Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430]")
Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430]
Immunocytochemistry/Immunofluorescence: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunofluorescence analysis of NIH-3T3 cells using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430]](https://resources.bio-techne.com/images/products/Hsp47-Antibody-3K3H1-Immunohistochemistry-Paraffin-NBP3-15430-img0001.jpg "Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430]")
Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430]
Immunohistochemistry-Paraffin: Hsp47 Antibody (3K3H1) [NBP3-15430] - Immunohistochemistry of paraffin-embedded human colon using Hsp47 Rabbit mAb (NBP3-15430) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.Applications for Hsp47 Antibody (3K3H1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSP47
Long Name
Heat Shock Protein 47
Alternate Names
AsTP3, BERF-1, CBP1, CBP2, Colligin, gp46, OI10, PPROM, RA-A47, Serpin H1, SERPINH1
Gene Symbol
SERPINH1
Additional HSP47 Products
Product Documents for Hsp47 Antibody (3K3H1)
Product Specific Notices for Hsp47 Antibody (3K3H1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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