Hsp47 Antibody (M16.10A1)

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Western Blot: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of HSP47, mAb (M16.10A1): Lane 1: Rat recombinant HSP47; Lane 2: Rat-2 (heat shocked); Lane 3: L929 (heat shocked); Lane 4: 3T3 (heat shocked); Lane 5: CHO (heat shocked); Lane 6: HeLa (heat shocked).

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of paraffin-embedded tissue section of rat carotid artery 14 days after balloon-injury to the artery, stained using Hsp47 (Colligin) mAb (M16.10A1).

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Immunohistochemistry-Paraffin: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Analysis of human breast cancer tissue immunohistochemically stained using Hsp47 mAb (M16.10A1).

Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491]

Simple Western: Hsp47 Antibody (M16.10A1) [NBP1-97491] - Simple Western lane view shows a specific band for Hsp47 in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta-catenin revealed nuclear staining of beta-catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] -

Immunocytochemistry/ Immunofluorescence: Hsp47 Antibody (M16.10A1) [NBP1-97491] - WNT & BMP signaling pathways play crucial roles in regeneration. a–d Real-time PCR for WNT & BMP signals, including Wnt7b (a), Wnt10a (b), Lef1 (c), & Bmp2 (d) in response to stretch (day 1 & day 7) & release of stretch (day 9); n = 6 for each group. e Schematic of hair cycle activator & inhibitor in response to strain alteration. f Dual immunostaining for K15 & beta-catenin revealed nuclear staining of beta-catenin in hair follicles at day 9 & day 14 when stretch was released. Scale bar = 50 μm. g Dual immunostaining for K15 & phospho-Smad1/5 (pSmad1/5) revealed the nuclear staining of pSmad1/5 in hair stem cells at day 7 when skin was stretched (arrow). Scale bar = 50 μm. h, i Immunostaining (h) & confocal microscope (i) revealed co-localization of HSP47 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (h) or 10 μm (i). j, k Immunostaining (j) & confocal microscope (k) revealed co-localization of Perilipin-1 & BMP-2 signals at day 7 when skin was stretched. Scale bar = 100 μm (j) or 10 μm (k). Statistical significance was determined using ANOVA followed by a Bonferroni post hoc test. Data are presented as means ± SEM. *p < 0.05. **p < 0.01. ***p < 0.001. Source data are provided as a Source Data file. *Autofluorescence of hair shafts in g, h Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/30944305), licensed under a CC-BY license. Not internally tested by Novus Biologicals.