HSP60 Antibody (3G8) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37617

Novus Biologicals, part of Bio-Techne
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NBP2-37617
NBP2-37617-0.025ml
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey, Primate

Applications

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 3G8

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all HSP60 Antibodies »

Product Summary for HSP60 Antibody (3G8) - BSA Free

Immunogen

Purified recombinant fragment of human HSP60 expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP60 Antibody (3G8) - BSA Free

Western Blot: HSP60 Antibody (3G8)BSA Free [NBP2-37617]

Western Blot: HSP60 Antibody (3G8)BSA Free [NBP2-37617]

Western Blot: HSP60 Antibody (3G8) [NBP2-37617] - Western blot analysis using HSP60 mouse mAb against T47D (1), Hela (2), HepG2 (3), A549 (4), Jurkat (5), HEK293 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (3G8) - BSA Free [NBP2-37617]

Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (3G8) - BSA Free [NBP2-37617]

Immunocytochemistry/Immunofluorescence: HSP60 Antibody (3G8) [NBP2-37617] - Immunofluorescence analysis of 3T3-L1 cells using HSP60 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry: HSP60 Antibody (3G8) - BSA Free [NBP2-37617]

Immunohistochemistry: HSP60 Antibody (3G8) - BSA Free [NBP2-37617]

Immunohistochemistry: HSP60 Antibody (3G8) [NBP2-37617] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues (left) and colon cancer tissues (right) using HSP60 mouse mAb with DAB staining.
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Applications for HSP60 Antibody (3G8) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP60

Hsp60 is a member of a highly conserved family which includes molecular chaperones from several species such as plant Hsp60 (known as Rubisco binding protein), GroEL, the E.coli Hsp60 and 65 kDa major antigen of mycobacteria. In eukaryotes, Hsp60 is localized in the mitochondrial matrix and in plants Hsp60 is localized in the chloroplast. Mitochondria, chloroplasts and bacteria have a common ancestry (>1 billion years) and this fact together with the high degree of homology between the divergent Hsp60s would indicate that these proteins carry out a primitive but important function which is similar to all of these different species. The common characteristics of the Hsp60s from the divergent species are i) high abundance, ii) induction with environmental stress such as heat shock, iii) homo oligomeric structures of either 7 or 14 subunits which reversibly dissociate in the presence of magnesium ions and ATP, iv) ATPase activity and v) a role in folding and assembly of oligomeric protein structures. These similarities are supported by recent studies where the single ring human mitochondrial homolog, Hsp60 with its co chaperonin, Hsp10 were expressed in a E. coli strain, engineered so that the groE operon is under strict regulatory control. This study has demonstrated that expression of Hsp60-Hsp10 was able to carry out all essential in vivo functions of GroEL and its co chaperonin, GroES. Consistent with their functions as chaperones, Hsp60 and Hsp10 have been suggested to act as docking molecules with a passive role in the maturation of caspase processing. Data demonstrates that recombinant Hsp60 and Hsp10 have been shown to accelerate the activation of procaspase 3 by cytochrome c and dATP in an ATP dependent manner. Hsps are intracellular proteins which are thought to serve protective functions against infection and cellular stress, however several recent studies indicate that members of the Hsp60 family are linked to a number of autoimmune diseases, arthrosclerosis and chlamydial disease.

Long Name

Heat Shock Protein 60

Alternate Names

cpn60, GROEL, HSPD1, SPG13

Gene Symbol

HSPD1

Additional HSP60 Products

Product Documents for HSP60 Antibody (3G8) - BSA Free

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Product Specific Notices for HSP60 Antibody (3G8) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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