HSP60 Antibody (4B9/89) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-22440
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat, Primate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 4B9/89
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Human placental HSP60.
Marker
Mitochondria Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for HSP60 Antibody (4B9/89) - Azide and BSA Free
Western Blot: HSP60 Antibody (4B9/89)Azide and BSA Free [NBP2-22440]
Western Blot: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of 50ug of the indicated whole cell lysates and 15ul of PageRuler Prestained Protein Ladder.Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]
Immunocytochemistry/Immunofluorescence: HSP60 Antibody (4B9/89) [NBP2-22440] - Analysis of Heat Shock Protein 60 (HSP60) (green) in HeLa and NIH3T3 cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with a HSP60 monoclonal antibody, at a dilution of 1:50 for at least 1 hour at room temperature, washed with PBS, and incubated with DyLight 488 goat-anti-mouse IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. Nuclei (blue) were stained with Hoechst 33342 dye.Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) - Azide and BSA Free [NBP2-22440]
Immunohistochemistry-Paraffin: HSP60 Antibody (4B9/89) [NBP2-22440] - Normal deparaffinized human Tonsil tissue.Applications for HSP60 Antibody (4B9/89) - Azide and BSA Free
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:200
Immunohistochemistry-Paraffin
1:200
Immunoprecipitation
2 ug
Western Blot
1:100 - 1:1000
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Reconstitute with PBS.
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSP60
Long Name
Heat Shock Protein 60
Alternate Names
cpn60, GROEL, HSPD1, SPG13
Entrez Gene IDs
3329 (Human)
Gene Symbol
HSPD1
UniProt
Additional HSP60 Products
Product Documents for HSP60 Antibody (4B9/89) - Azide and BSA Free
Product Specific Notices for HSP60 Antibody (4B9/89) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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