HSP60 Antibody (LK2)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-32867
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat, Bacteria, S. pyogenes, Chicken, E. coli, Guinea Pig, Hamster, Invertebrate, Monkey, Parasite, Plant, Yeast
Cited:
Human, Mouse, Yeast
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Simple Western, Western Blot, Western Blot (Negative)
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # LK2
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Recombinant human HSP60 protein (Uniprot: P10809 )
Epitope
aa 383-419
Reactivity Notes
Borellia, Streptococcus pyogenes, Yersinia entercolitica, Salmonella typhimurium, Treponema hyodysenteriae, Treponema innocense, Trichinella spiralis, Helminths, and Spinach.
Localization
Mitochondrial
Specificity
Recognizes a 60kDa protein, identified as the heat shock protein 60 (HSP60). Its epitope is localized between aa 383-419 of human HSP60. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (hsp s), more appropriately called as stress response proteins (srp s). HSP60 is a potential antigen in a number of autoimmune diseases. In human arthritis and in experimentally induced arthritis in animals, disease development coincides with the development of immune reactivity directed against not only bacterial HSP60, but also against its mammalian homolog.
Marker
Mitochondrial Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
60 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP2-34671)
Antibody with azide - store at 2 to 8C.
Antibody with azide - store at 2 to 8C.
Scientific Data Images for HSP60 Antibody (LK2)
Simple Western: HSP60 Antibody (LK2) [NBP2-32867]
Simple Western: HSP60 Antibody (LK2) [NBP2-32867] - Simple Western lane view shows a specific band for HSP60 in 0.2 mg/ml of HeLa (left) and HepG2 (right) lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.Western Blot: HSP60 Antibody (LK2) [NBP2-32867]
Western Blot: HSP60 Antibody (LK2) [NBP2-32867] - Western blot analysis of Hep2 cell lysate using HSP60 Mouse Monoclonal Antibody (LK2).Immunocytochemistry/ Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867]
Immunocytochemistry/Immunofluorescence: HSP60 Antibody (LK2) [NBP2-32867] - Immunofluorescent staining of MEOH-fixed HeLa cells using followed by goat anti-Mouse labeled with CF488 (Green); Nuclei are labeled with NucSpot red).Applications for HSP60 Antibody (LK2)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry-Paraffin
1-2 ug/ml
Simple Western
1:10
Western Blot
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Optimal dilution for a specific application should be determined.
In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: HSP60
Long Name
Heat Shock Protein 60
Alternate Names
cpn60, GROEL, HSPD1, SPG13
Gene Symbol
HSPD1
UniProt
Additional HSP60 Products
Product Documents for HSP60 Antibody (LK2)
Product Specific Notices for HSP60 Antibody (LK2)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...