Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine

Applications

Flow (Cell Surface), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 1H11

Concentration

0.5 mg/ml

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

Human native HSP70 protein

Localization

Cell membrane

Specificity

Binds the altered conformation of HSP70 embedded in the cell membrane and to intracellular HSP70 to a lesser extent.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSP70/HSPA1A Antibody (1H11)

Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Western Blot: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis of Human Heat shocked HeLa cell lysates showing detection of HSP70 protein using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11. Lane 1: Molecular Weight ladder (MW). Lane 2: HeLa cell lysates. Load: 20 ug. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:1000.
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - Analysis on non-permeabilized HCT116 cells using Mouse Anti-HSP70 Monoclonal Antibody, Clone 1H11: FITC conjugate showing cell membrane staining. Tissue: HCT116 cells. Species: Human. Fixation: 4% Formaldehyde. Permeabilization: None. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody at 1:100. Counterstain: Wheat germ agglutinin Texas red membrane marker; DAPI (blue) nuclear stain. Localization: Cell surface, cell membrane. (A) DAPI nuclear stain. (B) Wheat germ agglutinin Texas red. (C) HSP70 Antibody. (D) Composite. Courtesy of: Lawrence Hightower, Charles Giardina, and Didem Ozcan from University of Connecticut.
Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339]

Flow (Cell Surface): HSP70/HSPA1A Antibody (1H11) [NBP2-59339] - A cell surface stain was performed on HeLa cells with Hsp70 / Hsc70 Antibody (1H11) NBP2-59339PE (blue) and a matched isotype control(orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Phycoerythrin.
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Applications for HSP70/HSPA1A Antibody (1H11)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (1H11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody (1H11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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