HSP70/HSPA1A Antibody (5U9A0)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16482
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5U9A0
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 441-641 of human HSP70/HSPA1A (P0DMV8). QVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSP70/HSPA1A Antibody (5U9A0)
![Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-5U9A0-Western-Blot-NBP3-16482-img0003.jpg "Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]")
Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]
Western Blot: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.![Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0002.jpg "Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]")
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]
Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of NIH-3T3 cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.![Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-5U9A0-Immunocytochemistry-Immunofluorescence-NBP3-16482-img0001.jpg "Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]")
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482]
Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody (5U9A0) [NBP3-16482] - Immunofluorescence analysis of HeLa cells using HSP70/HSPA1A Rabbit mAb (NBP3-16482) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for HSP70/HSPA1A Antibody (5U9A0)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSP70/HSPA1A
Long Name
Heat Shock Protein 70
Alternate Names
HSP70-1A, HSP72, HSPA1A
Gene Symbol
HSPA1A
Additional HSP70/HSPA1A Products
Product Documents for HSP70/HSPA1A Antibody (5U9A0)
Product Specific Notices for HSP70/HSPA1A Antibody (5U9A0)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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