HSP70/HSPA1A Antibody (OTI3G9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-02190

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine, Monkey, Primate

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3G9

Concentration

1 mg/ml

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human HSPA1A (NP_005336) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP70/HSPA1A Antibody (OTI3G9)

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Western Blot: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA1A (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA1A.(1:500)
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunocytochemistry/Immunofluorescence: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY Hsp70.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody (OTI3G9) [NBP2-02190]

Immunohistochemistry-Paraffin: Hsp70 Antibody (3G9) [NBP2-02190] - Staining of paraffin-embedded prostate tissue using anti-Hsp70 mouse monoclonal antibody.
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Applications for HSP70/HSPA1A Antibody (OTI3G9)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50

Western Blot

1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

This intronless gene encodes a 70kDa heat shock protein which is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody (OTI3G9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody (OTI3G9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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