HSP70/HSPA1A Antibody (PD01-65)

Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445]

Western Blot: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Western blot analysis of HSP70/HSPA1A on different lysates with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/1,000 dilution.

Lane 1: HCT 116 cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: A431 cell lysate
Lane 5: A549 cell lysate

Lysates/proteins at 20 ug/Lane.

Predicted band size: 70 kDa
Observed band size: 70 kDa

Exposure time: 24 seconds;

4-20% SDS-PAGE gel.

Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (NBP3-32445) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:100,000 dilution was used for 1 hour at room temperature.

Immunohistochemistry: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445]

Immunohistochemistry: HSP70/HSPA1A Antibody (PD01-65) [NBP3-32445] - Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Rabbit anti-HSP70/HSPA1A antibody (NBP3-32445) at 1/200 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32445) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.