HSP70/HSPA1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77455

Novus Biologicals, part of Bio-Techne
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NBP1-77455
NBP1-77455SS
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%), Porcine (100%), Primate (100%), Rat (92%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Simple Western

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen, In vivo assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.18 mg/ml

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal portion of the human Hsp70 protein (between residues 550-600)

Localization

Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]

Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.
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Applications for HSP70/HSPA1A Antibody - BSA Free

Application
Recommended Usage

Flow Cytometry

1 : 200

Immunocytochemistry/ Immunofluorescence

1:25

Immunohistochemistry

1:400

Immunohistochemistry-Frozen

reported in scientific literature (PMID 26755821)

Immunohistochemistry-Paraffin

1:400

Simple Western

1:50
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 3 using NBP1-77455 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.18 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Heat shock protein 70 1A/1B (also known as HSP70, HSPA1A, HSPA1B, HSP70 1/2) is a chaperone protein which can stabilize proteins against aggregation and assist in protein folding. It has been shown to play a role in aging, viral replication, lupus, and tumor progression. Accordingly, HSP70 has been used in anti-tumor vaccines, as well as HIV and Parkinson's research.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

UniProt

P08107

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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