HSP70/HSPA1A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77456

Novus Biologicals, part of Bio-Techne
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NBP1-77456
NBP1-77456SS
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Human, Mouse, Rat

Applications

Validated:

Block/Neutralize, Electron Microscopy, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Block/Neutralize, Electron Microscopy, Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all HSP70/HSPA1A Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminus portion of the human Hsp70 protein (between residues 600-641) [UniProt # P08107]

Reactivity Notes

Use in Human reported in scientific literature (PMID:33760187).

Localization

Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody - BSA Free

Western Blot: HSP70/HSPA1A AntibodyBSA Free [NBP1-77456]

Western Blot: HSP70/HSPA1A AntibodyBSA Free [NBP1-77456]

Western Blot: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of HSP70 in (1) human brain, (2) human liver, (3) human testes, (4) human skeletal muscle, and (5) human heart.
Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77456]

Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77456]

Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77456] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.
Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77456]

Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77456]

Simple Western: HSP70/HSPA1A Antibody [NBP1-77456] - Lane view shows a specific band for Hsp70 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.

Applications for HSP70/HSPA1A Antibody - BSA Free

Application
Recommended Usage

Block/Neutralize

reported in scientific literature (PMID 30417859)

Electron Microscopy

reported in scientific literature (PMID 33335563)

Flow Cytometry

reported in scientific literature (PMID 33760187)

Immunohistochemistry

1:400

Immunohistochemistry-Paraffin

1:400

Simple Western

1:50

Western Blot

1:1000
Application Notes
This Hsp70 antibody is useful for Western blot and IHC-paraffin embedded sections.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS and 30% Glycerol

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

UniProt

P08107

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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