HSP70/HSPA1A Antibody - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16090

Novus Biologicals, part of Bio-Techne
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NBP3-16090
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP70/HSPA1A Antibodies »

Product Summary for HSP70/HSPA1A Antibody - Azide and BSA Free

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human HSP70/HSPA1A (NP_005336.3). KITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP70/HSPA1A Antibody - Azide and BSA Free

Western Blot: HSP70/HSPA1A AntibodyAzide and BSA Free [NBP3-16090]

Western Blot: HSP70/HSPA1A AntibodyAzide and BSA Free [NBP3-16090]

Western Blot: HSP70/HSPA1A Antibody [NBP3-16090] - Western blot analysis of extracts of various cell lines, using HSP70/HSPA1A antibody (NBP3-16090) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 0.5s.
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - Azide and BSA Free [NBP3-16090]

Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - Azide and BSA Free [NBP3-16090]

Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP3-16090] - Immunofluorescence analysis of U2OS cells using HSP70/HSPA1A Rabbit pAb (NBP3-16090) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody - Azide and BSA Free [NBP3-16090]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody - Azide and BSA Free [NBP3-16090]

Immunohistochemistry-Paraffin: HSP70/HSPA1A Antibody [NBP3-16090] - Immunohistochemistry of paraffin-embedded human esophageal cancer using HSP70/HSPA1A Rabbit pAb (NBP3-16090) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
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Applications for HSP70/HSPA1A Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 50% glycerol, pH7.3

Format

Azide and BSA Free

Preservative

0.05% Proclin 300

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSP70/HSPA1A

Hsp70 (Heat Shock 70kDa Protein 1A) belongs to a family of conserved ubiquitously expressed heat shock proteins with similar structure in virtually all living organisms. Hsp70 has plays an important role of the cell's machinery for protein folding. Hsp70 is also involved in stabilizing proteins against aggregation and allows them to refold by binding to hydrophobic residues exposed by stress. Additionally, Hsp70 directly inhibits heat-induced apoptosis.

Long Name

Heat Shock Protein 70

Alternate Names

HSP70-1A, HSP72, HSPA1A

Gene Symbol

HSPA1A

Additional HSP70/HSPA1A Products

Product Documents for HSP70/HSPA1A Antibody - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP70/HSPA1A Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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