HSP90 Antibody (4B5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15145

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15145
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Key Product Details

Species Reactivity

Human, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP90 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human HSP90. UniProt ID (P07900).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HSP90 Antibody (4B5)

Western Blot: HSP90 Antibody (4B5) [NBP3-15145]

Western Blot: HSP90 Antibody (4B5) [NBP3-15145]

Western Blot: HSP90 Antibody (4B5) [NBP3-15145] - Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, K562 whole cell lysate, HL-60 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate, Jurkat whole cell lysate, PC3 whole cell lysate, Rat brain tissue
All lanes: HSP90 antibody at 0.8ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 85 KDa.
Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4B5) [NBP3-15145]

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4B5) [NBP3-15145]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4B5) [NBP3-15145] - Immunofluorescence staining of NIH/3T3 cells with at HSP90 antibody 1:26, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).
Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145]

Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145]

Immunohistochemistry: HSP90 Antibody (4B5) [NBP3-15145] - IHC image of HSP90 antibody diluted at 1:80 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Applications for HSP90 Antibody (4B5)

Application
Recommended Usage

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 150mM NaCl, and 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HSP90

Hsp90 has at least 2 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (4B5)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP90 Antibody (4B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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