HSP90 Antibody (4D1)

Western Blot: HSP90 Antibody (4D1) [NBP3-15147]

Western Blot: HSP90 Antibody (4D1) [NBP3-15147] - Positive WB detected in: Jurkat whole cell lysate. All lanes: HSP90 antibody at 1.9ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 85, 99 KDa. Observed band size: 90 KDa.

Immunocytochemistry/ Immunofluorescence: HSP90 Antibody (4D1) [NBP3-15147]

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (4D1) [NBP3-15147] - Immunofluorescence staining of NIH/3T3 cells with at HSP90 antibody 1:63, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry: HSP90 Antibody (4D1) [NBP3-15147]

Immunohistochemistry: HSP90 Antibody (4D1) [NBP3-15147] - IHC image of HSP90 antibody diluted at 1:190 and staining in paraffin-embedded human testis tissue. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.