HSP90 Antibody (AC88)

Novus Biologicals, part of Bio-Techne | Catalog # NB100-1972

Novus Biologicals, part of Bio-Techne
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NB100-1972-0.025mg
NB100-1972
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bacteria, Canine, Chicken, Fish, Guinea Pig, Primate, Rabbit, Sheep

Cited:

Human, Mouse

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # AC88

Concentration

1.0 mg/ml

View all HSP90 Antibodies »

Product Specifications

Immunogen

Achlya ambisexualis (water mold) Hsp90.

Epitope

The epitope of this antibody has been mapped to amino acid residues 604-697 of the human Hsp90 sequence.

Reactivity Notes

Recognizes Beluga, C. elegans, Fusobacterium nucleatum, Mycobacterium leprae, Mussel, Scallop and Water Mold HSP90. Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Localization

Cytoplasmic

Specificity

Recognizes both Hsp90 alpha and beta. It reacts with the rodent glucocorticoid receptor and with proliferation potential proteins (P2PS) with apparent molecular masses of 30-40 kDa.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP90 Antibody (AC88)

Western Blot: HSP90 Antibody (AC88) [NB100-1972]

Western Blot: HSP90 Antibody (AC88) [NB100-1972]

Western Blot: HSP90 Antibody (AC88) [NB100-1972] - Analysis of HSP90, mAb (AC88): Lane 1: MW Marker; Lane 2: HSP90 (human), (native) ; Lane 3: HSP90 beta, (human) (recombinant); Lane 4: HSP90a (human), (recombinant); Lane 5: HeLa (heat shocked); Lane 6: 3T3 (heat shocked); Lane 7: PC-12 (heat shocked) ; Lane 8: CHO-K1 (heat shocked).
Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972]

Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972]

Immunohistochemistry: HSP90 Antibody (AC88) [NB100-1972] - Analysis of human spleen tissue stained with HSP90, mAb (AC88) at 10 ug/ml.
Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972]

Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972]

Flow (Intracellular): HSP90 Antibody (AC88) [NB100-1972] - An intracellular stain was performed on HeLa cells with HSP90 Antibody (AC88) NB100-1972PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin. Image using the PE format of this antibody.
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Applications for HSP90 Antibody (AC88)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1 ug/ml
Application Notes
Use in ICC/IF reported in scientific literature (PMID: 30373228). Simple Western reported by internal validation: Tested in A549 response to heatshock; separated by size; antibody dilution of 1:50.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90

In mammalian cells there are at least two Hsp90 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steriod receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Entrez Gene IDs

3320 (Human); 15519 (Mouse); 299331 (Rat)

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (AC88)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP90 Antibody (AC88)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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