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HSP90 Antibody (S08-8A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15043

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15043-25ul
NBP3-15043-100ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S08-8A1

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant protein of human HSP90 (Uniprot#: P07900 P08238)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP90 Antibody (S08-8A1)

Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043]

Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043]

Western Blot: HSP90 Antibody (S08-8A1) [NBP3-15043] - Western blot detection of Hsp90 in Rat Brain, C6, 3T3, Hela cell lysates using Hsp90 Rabbit mAb (1:1000 diluted). Predicted band size: 85, 83kDa. Observed band size: 90kDa.

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (S08-8A1) [NBP3-15043] -

Immunocytochemistry/Immunofluorescence: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunofluorescence of Hsp90 (green) in Hela cells using NBP3-15043 at dilution 1/200, and DAPI (blue)
Immunohistochemistry-Paraffin: HSP90 Antibody (S08-8A1) [NBP3-15043]

Immunohistochemistry-Paraffin: HSP90 Antibody (S08-8A1) [NBP3-15043]

Immunohistochemistry-Paraffin: HSP90 Antibody (S08-8A1) [NBP3-15043] - Immunohistochemistry of HSP90 in paraffin-embedded Human tonsil using HSP90 Rabbit mAb at dilution 1/5

Applications for HSP90 Antibody (S08-8A1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Immunohistochemistry

1/200

Western Blot

1/2000-1/10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90

Hsp90 has at least 2 isoforms, Hsp90a and Hsp90b which are encoded by separate genes. These ubiquitous and highly conserved proteins account for 1-2% of all cellular proteins in most cells. Hsp90 is part of the cells powerful network of chaperones to fight the deleterious consequences of protein unfolding caused by nonphysiological conditions. However, in the absence of stress, Hsp90 is a necessary component of fundamental cellular processes such as hormone signaling and cell cycle control. In this context several key regulatory proteins such as steroid receptors, cell cycle kinases involved in signal transduction and p53 have been identified as substrates of Hsp90. It has been suggested that Hsp90 acts as a capacitor for morphological evolution by buffering widespread variation, which may affect morphogenic pathways.

Long Name

Heat Shock Protein 90

Alternate Names

FLJ31884, Heat shock 86 kDa, heat shock 90kD protein 1, alpha, heat shock 90kD protein 1, alpha-like 4, heat shock 90kD protein, alpha-like 4, heat shock 90kDa protein 1, alpha, heat shock protein 90kDa alpha (cytosolic), class A member 1, heat shock protein HSP 90-alpha, Hsp89, Hsp90, HSP90AHSP86, HSP90N, HSPC1HSP 86, HSPCAHSP89A, HSPCAL1, HSPCAL4, HSPN, LAP2, Renal carcinoma antigen NY-REN-38

Gene Symbol

HSP90AA1

Additional HSP90 Products

Product Documents for HSP90 Antibody (S08-8A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSP90 Antibody (S08-8A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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