HSP90 beta Antibody (5G4) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37590

Novus Biologicals, part of Bio-Techne
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NBP2-37590
NBP2-37590-0.025ml
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Monkey, Primate

Applications

Validated:

ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5G4

Format

BSA Free

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all HSP90 beta Antibodies »

Product Specifications

Immunogen

Purified recombinant fragment of human HSP90 beta expressed in E. Coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

84 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSP90 beta Antibody (5G4) - BSA Free

Western Blot: HSP90 beta Antibody (5G4)BSA Free [NBP2-37590]

Western Blot: HSP90 beta Antibody (5G4)BSA Free [NBP2-37590]

Western Blot: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis using HSP90AB1 mouse mAb against Jurkat (1), A431 (2), Hela (3), A549 (4), HEK293 (5), K562 (6), NIH/3T3 (7), PC-12 (8) and Cos7 (9) cell lysate.
Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (5G4) - BSA Free [NBP2-37590]

Immunocytochemistry/ Immunofluorescence: HSP90 beta Antibody (5G4) - BSA Free [NBP2-37590]

Immunocytochemistry/Immunofluorescence: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of Hela cells using HSP90AB1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) - BSA Free [NBP2-37590]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) - BSA Free [NBP2-37590]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (5G4) [NBP2-37590] - Analysis of paraffin-embedded kidney cancer tissues (left) and brain tissues (right) using HSP90AB1 mouse mAb with DAB staining.
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Applications for HSP90 beta Antibody (5G4) - BSA Free

Application
Recommended Usage

ELISA

1:10000

Flow Cytometry

1:200 - 1:400

Immunocytochemistry/ Immunofluorescence

1:200 - 1:1000

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Ascites

Format

BSA Free

Preservative

0.03% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (5G4) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP90 beta Antibody (5G4) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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