HSP90 beta Antibody (HSP90AB1/3953)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07192

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HSP90AB1/3953

Concentration

0.2 mg/ml

View all HSP90 beta Antibodies »

Product Summary for HSP90 beta Antibody (HSP90AB1/3953)

Immunogen

Recombinant human HSP90 beta protein fragment (around aa581-704) (exact sequence is proprietary) (Uniprot: P08238)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0 mg/ml. (NBP3-08908)

Antibody with azide - store at 2 to 8C.

Scientific Data Images for HSP90 beta Antibody (HSP90AB1/3953)

Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Western Blot: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Western blot analysis of MCF-7 cell lysate using HSP90 beta antibody (HSP90AB1/3953).
Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).
Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192]

Protein Array: HSP90 beta Antibody (HSP90AB1/3953) [NBP3-07192] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).

Applications for HSP90 beta Antibody (HSP90AB1/3953)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

UniProt

P08238

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (HSP90AB1/3953)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSP90 beta Antibody (HSP90AB1/3953)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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