Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Protein Array, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HSP90AB1/3953

Format

Azide and BSA Free

Concentration

1 mg/ml

View all HSP90 beta Antibodies »

Product Specifications

Immunogen

Recombinant human HSP90 beta protein fragment (around aa581-704) (exact sequence is proprietary) (Uniprot: P08238)

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

90 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA. Also available at 200 ug/ml WITH BSA & azide (NBP3-07192).

Antibody with azide - store at 2 to 8C.

Scientific Data Images for HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free

Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908]

Immunohistochemistry-Paraffin: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908] - Formalin-fixed, paraffin-embedded human prostate stained with HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).
Western Blot: HSP90 beta Antibody (HSP90AB1/3953)Azide and BSA Free [NBP3-08908]

Western Blot: HSP90 beta Antibody (HSP90AB1/3953)Azide and BSA Free [NBP3-08908]

Western Blot: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908] - Western blot analysis of MCF-7 cell lysate using HSP90 beta Mouse Monoclonal Antibody (HSP90AB1/3953).
Protein Array: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908]

Protein Array: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908]

Protein Array: HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free [NBP3-08908] - Analysis of Protein Array containing more than 19,000 full-length human proteins using HSP90 beta Mouse Monoclonal Antibody (HSP90 beta/3953).

Applications for HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml

Western Blot

1-2 ug/ml
Application Notes
Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HSP90 beta

The 90-kDa heat shock protein (Hsp90) is a highly conserved and abundant cytosolic homodimeric molecular chaperone (1). Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine, serine/threonine kinases and actin (2-3). Two isoforms which correspond to the major and minor isoform, (Hsp90-

Long Name

Heat Shock Protein 90 beta

Alternate Names

D6S182, HSP84, HSP90AB1, HSP90B, HSPC2, HSPCB

Gene Symbol

HSP90AB1

Additional HSP90 beta Products

Product Documents for HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HSP90 beta Antibody (HSP90AB1/3953) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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