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HspA4 Antibody (10A9C5)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15869

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15869-100ul
NBP3-15869-20ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10A9C5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for HspA4 Antibody (10A9C5)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 700-800 of human HspA4 (P34932). EELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HspA4 Antibody (10A9C5)

Western Blot: HspA4 Antibody (10A9C5) [NBP3-15869]

Western Blot: HspA4 Antibody (10A9C5) [NBP3-15869]

Western Blot: HspA4 Antibody (10A9C5) [NBP3-15869] - Western blot analysis of extracts of various cell lines, using HspA4 Rabbit mAb (NBP3-15869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]

Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]

Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869] - Immunohistochemistry of paraffin-embedded mouse spinal cord using HspA4 Rabbit mAb (NBP3-15869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]

Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]

Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869] - Immunohistochemistry of paraffin-embedded rat liver using HspA4 Rabbit mAb (NBP3-15869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for HspA4 Antibody (10A9C5)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HspA4

Alternate Names

APG2, APG-2, heat shock 70 kDa protein 4, heat shock 70kD protein 4, heat shock 70kDa protein 4, Heat shock 70-related protein APG-2, heat shock protein, 110 kDa, HS24/P52, hsp70, hsp70RY, MGC131852, RY

Gene Symbol

HSPA4

Additional HspA4 Products

Product Documents for HspA4 Antibody (10A9C5)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HspA4 Antibody (10A9C5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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