HspA4 Antibody (10A9C5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15869
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 10A9C5
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for HspA4 Antibody (10A9C5)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 700-800 of human HspA4 (P34932). EELGKQIQQYMKIISSFKNKEDQYDHLDAADMTKVEKSTNEAMEWMNNKLNLQNKQSLTMDPVVKSKEIEAKIKELTSTCSPIISKPKPKVEPPKEEQKNA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HspA4 Antibody (10A9C5)
Western Blot: HspA4 Antibody (10A9C5) [NBP3-15869]
Western Blot: HspA4 Antibody (10A9C5) [NBP3-15869] - Western blot analysis of extracts of various cell lines, using HspA4 Rabbit mAb (NBP3-15869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]
Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869] - Immunohistochemistry of paraffin-embedded mouse spinal cord using HspA4 Rabbit mAb (NBP3-15869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869]
Immunohistochemistry-Paraffin: HspA4 Antibody (10A9C5) [NBP3-15869] - Immunohistochemistry of paraffin-embedded rat liver using HspA4 Rabbit mAb (NBP3-15869) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.Applications for HspA4 Antibody (10A9C5)
Application
Recommended Usage
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HspA4
Alternate Names
APG2, APG-2, heat shock 70 kDa protein 4, heat shock 70kD protein 4, heat shock 70kDa protein 4, Heat shock 70-related protein APG-2, heat shock protein, 110 kDa, HS24/P52, hsp70, hsp70RY, MGC131852, RY
Gene Symbol
HSPA4
Additional HspA4 Products
Product Documents for HspA4 Antibody (10A9C5)
Product Specific Notices for HspA4 Antibody (10A9C5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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