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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Rabbit, Sheep

Applications

Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Alexa Fluor 350 (Excitation = 346 nm, Emission = 442 nm)

Antibody Source

Monoclonal Rat IgG2A Clone # 1B5

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Native hamster Hsc70 (Hsp73).

Reactivity Notes

Turtle (100%).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350]

HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350]

HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350] [NBP1-97868AF350] -

HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350] [NBP1-97868AF350] - Vial of Alexa Fluor 350 conjugated antibody. Alexa Fluor 350 is optimally excited at 346 nm by the UV laser (350 or 355 nm) and has an emission maximum of 442 nm.

Applications for HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Electron Microscopy

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HSPA8/HSC71

Hsp73 is a normal cell constituent which is synthesized in increased abundance under adverse environmental circumstances, such as non-physiological temperatures or treatment with amino acid analogues (1). Data has shown a molecular mechanism for cytokine-mediated posttranscriptional regulation of Bim mRNA by Hsc70, which binds to AU-rich elements (AREs) in the 3'-untranslated region of specific mRNAs and enhances their stability. The RNA binding potential of Hsc70 is regulated by cochaperones including Bag-4 (also SODD), CHIP, Hip, and Hsp40 (2). Hsp73 protein expression was detected in the cytoplasm of melanoma cells in primary tumors and metastases. In primary melanomas, the proportion of Hsp73 protein expressing cells correlated with tumor thickness which was found to be statistically significant (3).

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (1B5) [Alexa Fluor® 350]



Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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