Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Bovine, Canine, Chicken, Guinea Pig, Hamster, Monkey, Rabbit, Sheep

Cited:

Human

Applications

Electron Microscopy, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 1B5

Concentration

1.0 mg/ml

View all HSPA8/HSC71 Antibodies »

Product Specifications

Immunogen

Native hamster Hsc70 (Hsp73).

Reactivity Notes

Turtle (100%).

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Theoretical MW

73 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (1B5)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of HSC70/HSP73: Lane 1: MW marker, Lane 2: HSC70/HSP73 (bovine), (recombinant) Lane 3: HSP70/HSP72 (human), (recombinant) Lane 4: 3T3, (Cell Lysate) Lane 5: PC-12, (Cell Lysate), Lane 6: CHO-K1 Cell Lysate, Lane 7: GPC-16 Cell Lysate, Lane 8: HeLa, (Cell Lysate).
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody (1B5) [NBP1-97868] - Analysis of human spleen tissue stained with HSC70/HSP73, mAb (1B5) at 10ug/ml.

Applications for HSPA8/HSC71/Hsc70 Antibody (1B5)

Application
Recommended Usage

Electron Microscopy

1:10-1:500

Flow Cytometry

1:10-1:1000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

1:10-1:500

Simple Western

reported by internal validation

Western Blot

1:1000
Application Notes
In Simple Western internal validation: Rat B103 cells as sample; separated by size; antibody dilution of 1:25; matrix was 12-230 kDa; detected by Chemiluminescence.

Reviewed Applications

Read 1 review rated 4 using NBP1-97868 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (1B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (1B5)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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