HSPA8/HSC71/Hsc70 Antibody (5B7)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97454

Novus Biologicals, part of Bio-Techne
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NBP1-97454-0.025mg
NBP1-97454-0.05mg
NBP1-97454-0.2mg
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Key Product Details

Species Reactivity

Validated:

Plant

Cited:

Plant

Applications

Validated:

Immunoprecipitation, Western Blot

Cited:

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 5B7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSPA8/HSC71 Antibodies »

Product Specifications

Immunogen

Recombinant spinach Hsc70 (Hsp73).

Reactivity Notes

Recognizes Plant HSC70.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody (5B7)

Western Blot: HSPA8/HSC71/Hsc70 Antibody (5B7) [NBP1-97454]

Western Blot: HSPA8/HSC71/Hsc70 Antibody (5B7) [NBP1-97454]

Western Blot: HSC70 Antibody (5B7) [NBP1-97454] - Analysis of HSC70: Lane 1: MW marker, Lane 2: DnaK Recombinant E. coli Protein, Lane 3: Banana Lysate, Lane 4: Orange Lysate, Lane 5: Carrot Lysate, Lane 6: Cytosolic wheat HSC70.

Applications for HSPA8/HSC71/Hsc70 Antibody (5B7)

Application
Recommended Usage

Immunoprecipitation

1:10-1:500

Western Blot

1:100-1:2000
Application Notes
This antibody is useful in Immunoprecipitation and detects a band of ~70kDa by Western blot.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

The Hsp70 family of heat shock protiens contains multiple homologs ranging in size from 66-78 kDa, and are the eukaryotic equivalents of the bacterial DnaK. The most studied Hsp70 members include the cytosolic stress-induced Hsp70 (Hsp72), the constitutive cytosolic Hsc70 (Hsp73), and the ER-localized BiP (Grp78). Hsp70 family members contain highly conserved N-terminal ATP-ase and C-terminal protein binding domains. Binding of peptide to Hsp70 is assisted by Hsp40, and stimulates the inherent ATPase activity of Hsp70, facilitating ATP hydrolysis and enhanced peptide binding. Hsp70 nucleotide exchange and substrate binding coordinates the folding of newly synthesized proteins, the re-folding of misfolded or denatured proteins, coordinates trafficking of proteins across cellular membranes, inhibits protein aggregation, and targets the degradation of proteins via the proteasomal pathway.

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Gene Symbol

HSPA8

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody (5B7)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody (5B7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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