HSPA8/HSC71/Hsc70 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-41377

Novus Biologicals, part of Bio-Techne
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NB100-41377
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Canine (100%), Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA, Western Blot

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all HSPA8/HSC71 Antibodies »

Product Specifications

Immunogen

Peptide with sequence C-EKLQGKINDEDKQK corresponding to internal region according to NP_006588.1.

Specificity

This antibody is expected to recognise isoform 1 (NP_006588.1) only.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for HSPA8/HSC71/Hsc70 Antibody

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Western Blot: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of HepG2 (A) and NIH3T3 (B) cell lysate (35 ug protein in RIPA buffer). Antibody at 0.03ug/mL. Detected by chemiluminescence.
Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Immunocytochemistry/ Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Immunocytochemistry/Immunofluorescence: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (4 ug/mL).
Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377]

Immunohistochemistry-Paraffin: HSPA8/HSC71/Hsc70 Antibody [NB100-41377] - Staining of paraffin embedded Human Prostate. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for HSPA8/HSC71/Hsc70 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 ug/mL

Immunohistochemistry-Paraffin

5 ug/mL

Peptide ELISA

Detection limit 1:4000

Western Blot

0.03 - 0.1 ug/mL
Application Notes
Western blot: Approx 75 kDa band observed in lysates of cell lines HepG2 and NIH3T3 (calculated MW of 70.9 kDa according to Human NP_006588.1 and Mouse NP_112442.2). This molecular weight is routinely observed by other sources.
Immunofluorescence: Strong expression of the protein seen in the cytoplasm of HeLa cells. Use in FLOW reported in scientific literature (PMID: 30651550).

Reviewed Applications

Read 1 review rated 5 using NB100-41377 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: HSPA8/HSC71

The product encoded by this gene belongs to the heat shock protein 70 family which contains both heat-inducible and constitutively expressed members. The latter are called heat-shock cognate proteins. This gene encodes a heat-shock cognate protein. This protein binds to nascent polypeptides to facilitate correct folding. It also functions as an ATPase in the disassembly of clathrin-coated vesicles during transport of membrane components through the cell. Two alternatively spliced variants have been characterized to date.

Long Name

Heat Shock 70 kDa Protein 8

Alternate Names

HSC54, HSC70, HSC71, HSP71, HSP73, HSPA10, NIP71

Entrez Gene IDs

3312 (Human); 15481 (Mouse); 24468 (Rat)

Gene Symbol

HSPA8

UniProt

NP_006588.1 (Human)

Additional HSPA8/HSC71 Products

Product Documents for HSPA8/HSC71/Hsc70 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSPA8/HSC71/Hsc70 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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