HSV Tag Antibody [HRP]
Novus Biologicals, part of Bio-Techne | Catalog # NB600-515
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry (Negative), Western Blot
Label
HRP
Antibody Source
Polyclonal Goat IgG
Concentration
1.0 mg/ml
Product Summary for HSV Tag Antibody [HRP]
Immunogen
Goats were immunized with HSV (SQPELAPEDPED) conjugated to KLH. Antibody was isolated by affinity chromatography using the peptide immobilized on solid support.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for HSV Tag Antibody [HRP]
Western Blot: HSV Tag Antibody [HRP] [NB600-515] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used at 0.04 ug/ml (1:25,000).
Applications for HSV Tag Antibody [HRP]
Application
Recommended Usage
ELISA
1:10000 - 1:100000
Immunocytochemistry/ Immunofluorescence
1:200-1:500
Western Blot
1:1000-1:10000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Suggested starting dilutions are as follows. ELISA - 1:10,000-1:100,000 <br>Western Blots Colorimetric detection: 1:1,000 - 1:10,000 Chemiluminescent detection: 1:1,000 - 1:30,000 <br>Immunocytochemistry: 1:200 - 1:500 In some cases, the antibody may be diluted further than indicated.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 0.2% BSA
Preservative
0.05% Pro-Clean 400
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HSV Tag
Alternate Names
HSV epitope tag
Additional HSV Tag Products
Product Specific Notices for HSV Tag Antibody [HRP]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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