HTF9C Antibody (OTI1C3)
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46015
![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0004.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Western Blot: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Western-Blot-NBP2-46015-img0010.jpg "Western Blot: HTF9C Antibody (OTI1C3) [NBP2-46015]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # OTI1C3
Concentration
1 mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human TRMT2A(NP_073564) produced in HEK293T cell.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
68.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for HTF9C Antibody (OTI1C3)
Western Blot: HTF9C Antibody (OTI1C3) [NBP2-46015]
Western Blot: HTF9C Antibody (OTI1C3) [NBP2-46015] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HTF9C.![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0009.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]
Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015] - Analysis of Human Kidney tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)![Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Flow-Cytometry-NBP2-46015-img0002.jpg "Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015]
Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015] - Analysis of HEK293T cells transfected with either overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by TRMT2A antibody.![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0005.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0006.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0007.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunohistochemistry-NBP2-46015-img0008.jpg "Immunohistochemistry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Flow-Cytometry-NBP2-46015-img0001.jpg "Flow Cytometry: HTF9C Antibody (OTI1C3) [NBP2-46015]")
![Immunofluorescence: HTF9C Antibody (OTI1C3) [NBP2-46015]](https://resources.bio-techne.com/images/products/HTF9C-Antibody-OTI1C3-Immunofluorescence-NBP2-46015-img0003.jpg "Immunofluorescence: HTF9C Antibody (OTI1C3) [NBP2-46015]")
Applications for HTF9C Antibody (OTI1C3)
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Immunohistochemistry
1:150
Western Blot
1:200-2000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HTF9C
Alternate Names
HTF9CHpaII tiny fragments locus 9c protein, MGC102728, TRM2 tRNA methyltransferase 2 homolog A (S. cerevisiae), tRNA (uracil-5-)-methyltransferase homolog A, tRNA methyltransferase 2 homolog A
Gene Symbol
TRMT2A
Additional HTF9C Products
Product Documents for HTF9C Antibody (OTI1C3)
Product Specific Notices for HTF9C Antibody (OTI1C3)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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