Key Product Details

Species Reactivity

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 196C429

Concentration

0.5 mg/ml

View all HTRA2/Omi Antibodies »

Product Specifications

Immunogen

This monoclonal antibody was developed by immunizing mice with a synthetic peptide containing amino acids 335-350 of human HtrA2.

Specificity

This antibody does not cross-react with mouse HtrA2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for HTRA2/Omi Antibody (196C429)

Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]

Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558]

Western Blot: HTRA2/Omi Antibody (196C429) [NB100-56558] - Detection of HtrA2 using an HtrA2 monoclonal antibody. HL-60 cell lysate was probed with HtrA2 antibody at 3 ug/ml and two protein bands of approximate molecular weight of 50 and 38 kDa were detected. The 50 kDa and 38 kDa bands may represent precursor and mature forms of HtrA2.
Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]

Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558]

Immunohistochemistry: HTRA2/Omi Antibody (196C429) [NB100-56558] - Analysis of HtrA2 using an HtrA2 monoclonal antibody. Probing with HtrA2 antibody shows staining in stomach tumor tissue (A) and very weak staining in normal stomach tissue (B).

Applications for HTRA2/Omi Antibody (196C429)

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

reported in scientific literature (Lee et al (2003))

Western Blot

2-3 ug/ml

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS containing 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HTRA2/Omi

The inhibitor of apoptosis (IAP) family of proteins regulates programmed cell death triggered by various stimuli. All IAPs have at least one baculovirus IAP repeat (BIR) motif that is essential for their anti-apoptotic activity. Recently, a serine protease has been isolated, which is released from the mitochondria upon induction of apoptosis by apoptotic stimuli. This protein called Omi/HtrA2 is a mitochondrial protein that binds to IAP. Like Smac/DIABLO, the mature Omi protein contains a conserved IAP-binding motif (AVPS) at its N terminus. The deregulated expression of Omi in the cytoplasm of mammalian cells induces apoptosis indicating that Omi could participate in the mitochondrial apoptotic pathway.

Long Name

High Temperature Requirement Protein-2

Alternate Names

Omi, PRSS25

Gene Symbol

HTRA2

Additional HTRA2/Omi Products

Product Documents for HTRA2/Omi Antibody (196C429)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HTRA2/Omi Antibody (196C429)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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