BG01V human embryonic stem cells were stained with Mouse Anti-Human 5T4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB49751F, filled histogram) or isotype control antibody (Catalog # IC002F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of 5T4 in MCF-7 Human Cells by Flow Cytometry.

MCF-7 human breast cancer cell line was stained with Mouse Anti-Human 5T4 Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB49751F, filled histogram) or isotype control antibody (IC002F, open histogram). Staining was performed using our Staining Membrane-associated Proteins protocol.

Applications for Human 5T4 Fluorescein-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: BG01V human embryonic stem cells and MCF-7 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: 5T4

Human 5T4 (oncofetal antigen 5T4; also TPBG and trophoblast glycoprotein) is a 72 kDa glycoprotein member of the LRR family of proteins. It is expressed on trophoblasts, tumor cells, ovarian cuboidal epithelium and embryonic stem cells, and impacts cell adhesion and motility. The human 5T4 cDNA encodes a type I transmembrane protein precursor that is 420 amino acids (aa) in length. It contains a 324 aa extracellular region (aa 32‑355) that shows one Ser‑rich region followed by seven Leu-rich repeats (aa 90‑355). Over aa 31‑355, human 5T4 shares 81% and 85% aa identity with mouse and canine 5T4, respectively.

Long Name

Oncofetal Antigen/Trophoblast Glycoprotein

Alternate Names

M6P1, TPBG

Entrez Gene IDs

7162 (Human); 21983 (Mouse); 83684 (Rat); 102132149 (Cynomolgus Monkey)

Gene Symbol

TPBG

UniProt

Q13641

Additional 5T4 Products

Product Documents for Human 5T4 Fluorescein-conjugated Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human 5T4 Fluorescein-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human 5T4 Fluorescein-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB49751F

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all 5T4 Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human 5T4 Fluorescein-conjugated Antibody

Detection of 5T4 in BG01V Human Cells by Flow Cytometry.