Human A33 Antibody

Name: Human A33 Antibody
Brand: R&D Systems
SKU: MAB30802

Bio-Techne includes R&D Systems | Catalog # MAB30802

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Product Datasheet / COA / SDS

New Version Available - MAB3080

Discontinued Product

MAB30802 has been discontinued and is replaced by MAB3080.

Product Summary
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human

Applications

Simple Western, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 1053809

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Product Summary for Human A33 Antibody

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Specificity

Detects human A33 in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human A33 Antibody

Detection of Human A33 by Western Blot.

Western blot shows lysates of HT-29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human A33 Monoclonal Antibody (Catalog # MAB30802) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for A33 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Detection of Human A33 by Simple Western^TM.

Simple Western lane view shows lysates of HT-29 human colon adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for A33 at approximately 40 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human A33 Monoclonal Antibody (Catalog # MAB30802) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human A33 Antibody

Application

Recommended Usage

Simple Western

50 µg/mL
Sample: Lysates of HT-29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line

Western Blot

2 µg/mL
Sample: HT-29 human colon adenocarcinoma cell line and COLO 205 human colorectal adenocarcinoma cell line.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM-A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol