Human A33 Biotinylated Antibody

Name: Human A33 Biotinylated Antibody
Brand: R&D Systems
SKU: BAF3080
Rating: 5 (1 reviews)

R&D Systems, part of Bio-Techne | Catalog # BAF3080

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Product Datasheet / COA / SDS

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Conjugate

Catalog #

Unconjugated

AF3080

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Western Blot

Label

Biotin

Antibody Source

Polyclonal Goat IgG

View all A33 Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human A33
Ile22-Val235
Accession # Q99795

Specificity

Detects human A33 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant mouse A33 is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human A33 Biotinylated Antibody

Detection of A33 in HT‑29 Human Cell Line by Flow Cytometry.

HT-29 human colon carcinoma cell line was stained with Goat Anti-Human A33 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF3080, filled histogram) or isotype control antibody (Catalog # BAF108, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Applications for Human A33 Biotinylated Antibody

Application

Recommended Usage

Flow Cytometry

0.25 µg/10⁶ cells
Sample: HT‑29 human colon carcinoma cell line

Western Blot

0.1 µg/mL
Sample: Recombinant Human A33

Reviewed Applications

Read 1 review rated 5 using BAF3080 in the following applications:

Western Blot (1 Review)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: A33

Human A33, also known as GPA33, is a 43 kDa type I transmembrane glycoprotein that belongs to the CTX (cortical thymocyte marker in Xenopus) family of cell adhesion molecules within the immunoglobulin superfamily. Other family members include CXADR, ESAM, BT-IgSF, CD2 and JAM A-C. The extracellular domain (ECD) of human A33 is 214 amino acids (aa) in length and contains one V-type and one C2-type Ig-like domain. This ECD is 80%, 74% and 71% aa identical to canine, bovine and mouse A33 ECD, respectively. A33 is likely to be involved in cell-cell adhesion between epithelial cells.

Long Name

Cell Surface A33 Antigen

Alternate Names

GPA33

Entrez Gene IDs

10223 (Human); 59290 (Mouse)

Gene Symbol