Human Acetylated p53 (K382) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB13552
Recombinant Monoclonal Antibody.
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry, Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2589A
Product Specifications
Immunogen
Synthetic peptide containing acetylated human p53 (K382)
Accession # P04637
Accession # P04637
Specificity
Detects human acetylated p53 (K382) in Western blots.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Acetylated p53 (K382) Antibody
Detection of Human Acetylated p53 (K382) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with doxorubicin and trichostatin A (TSA). PVDF membrane was probed with 4 µg/mL of Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (HAF008). A specific band was detected for acetylated p53 (K382) at approximately 53 kDa (as indicated). GAPDH (MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.
Acetylated p53 (K382) in Lung Cancer.
Acetylated p53 (K382) was detected in immersion fixed paraffin-embedded sections of lung cancer tissue using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Acetylated p53 (K382) in HeLa Human Cell Line.
Acetylated p53 (K382) was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line treated with trichostatin A (TSA) and doxorubicin (left panel; positive staining) and untreated HeLa cell line (right panel; negative control) using Rabbit Anti-Human Acetylated p53 (K382) Monoclonal Antibody (Catalog # MAB13552) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to nuclei. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human Acetylated p53 (K382) Antibody
Application
Recommended Usage
Immunocytochemistry
3-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)
Immunohistochemistry
3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of lung cancer tissue
Sample: Immersion fixed paraffin-embedded sections of lung cancer tissue
Western Blot
4 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)
Sample: HeLa human cervical epithelial carcinoma cell line treated with doxorubicin and trichostatin A (TSA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p53
Alternate Names
BCC7, LFS1, TP53, TRP53
Gene Symbol
TP53
UniProt
Additional p53 Products
Product Documents for Human Acetylated p53 (K382) Antibody
Product Specific Notices for Human Acetylated p53 (K382) Antibody
For research use only
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