Human Activin B Precursor Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11450

R&D Systems, part of Bio-Techne
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MAB11450-100
MAB11450-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 132807

View all Activin B Antibodies »

Product Specifications

Immunogen

DNA construct encoding human Activin A pro region fused to mature human Activin beta B chain

Specificity

Detects human Activin A Precursor in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Activin B Precursor Antibody

Detection of Activin B in A375 cells (positive) and U266 cells (negative).

Activin B was detected in 10% Formalin fixed A375 human melanoma cell line (Positive) and absent in U266 human myeloma cell line (Negative) using Mouse Anti-Human Activin B Precursor Monoclonal Antibody (Catalog # MAB11450) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (Blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Activin B in PC-3 cells (positive) and U266 cells (negative).

Activin B was detected in 10% Formalin fixed PC-3 human prostate cancer cell line (Positive) and absent in U266 human myeloma cell line (Negative) using Mouse Anti-Human Activin B Precursor Monoclonal Antibody (Catalog # MAB11450) at 8 µg/ml for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (Blue). Specific staining was localized to cytoplasmic. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Activin B Precursor Antibody

Application
Recommended Usage

Immunocytochemistry

8 µg/mL
Sample: 10% Formalin fixed A375 human melanoma cell line (Positive), PC-3 human prostate cancer cell line (Positive) and U266 human myeloma cell line (Negative)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin B

Activin and Inhibin are members of the TGF-beta superfamily of cytokines and are involved in a wide range of biological processes including tissue morphogenesis and repair, fibrosis, inflammation, neural development, hematopoiesis, reproductive system function, and carcinogenesis (1‑7). Activin and Inhibin are produced as precursor proteins. Their amino terminal propeptides are proteolytically cleaved and facilitate formation of disulfide-linked dimers of the bioactive proteins (8, 9). Activins are nonglycosylated homodimers or heterodimers of various beta subunits ( betaA, betaB, betaC, and betaE in mammals), while Inhibins are heterodimers of a unique alpha subunit and one of the beta subunits. Activin A is a widely expressed homodimer of two betaA chains. The betaA subunit can also heterodimerize with a betaB or betaC subunit to form Activin AB and Activin AC, respectively (10). The 14 kDa mature human betaA chain shares 100% amino acid sequence identity with bovine, feline, mouse, porcine, and rat betaA. Activin A exerts its biological activities by binding to the type 2 serine/threonine kinase Activin RIIA which then noncovalently associates with the type 1 serine/threonine kinase Activin RIB/ALK-4 (7, 11). Signaling through this receptor complex leads to Smad activation and regulation of activin-responsive gene transcription (7, 11). The bioactivity of Activin A is regulated by a variety of mechanisms (11). BAMBI, Betaglycan, and Cripto are cell-associated molecules that function as decoy receptors or limit the ability of Activin A to induce receptor complex assembly (12‑14). The intracellular formation of Activin A can be prevented by the incorporation of the betaA subunit into Activin AC or Inhibin A (3, 10). And the bioavailability of Activin A is restricted by its incorporation into inactive complexes with alpha2-Macroglobulin, Follistatin, and FLRG (15, 16).

References

  1. Kumanov, P. et al. (2005) Reprod. Biomed. Online 10:786.
  2. Maeshima, A. et al. (2008) Endocr. J. 55:1.
  3. Rodgarkia-Dara, C. et al. (2006) Mutat. Res. 613:123.
  4. Werner, S. and C. Alzheimer (2006) Cytokine Growth Factor Rev. 17:157.
  5. Xu, P. and A.K. Hall (2006) Dev. Biol. 299:303.
  6. Shav-Tal, Y. and D. Zipori (2002) Stem Cells 20:493.
  7. Chen, Y.G. et al. (2006) Exp. Biol. Med. 231:534.
  8. Gray, A.M. and A.J. Mason (1990) Science 247:1328.
  9. Mason, A.J. et al. (1996) Mol. Endocrinol. 10:1055.
  10. Thompson, T.B. et al. (2004) Mol. Cell. Endocrinol. 225:9.
  11. Harrison, C.A. et al. (2005) Trends Endocrinol. Metab. 16:73.
  12. Onichtchouk, D. et al. (1999) Nature 401:480.
  13. Gray, P.C. et al. (2002) Mol. Cell. Endocrinol. 188:254.
  14. Kelber, J.A. et al. (2008) J. Biol. Chem. 283:4490.
  15. Phillips, D.J. et al. (1997) J. Endocrinol. 155:65.
  16. Schneyer, A. et al. (2003) Endocrinology 144:1671.

Long Name

Inhibin Subunit Beta B

Alternate Names

activin AB beta polypeptide, Activin Beta B, Activin beta-B chain, INHBB, Inhibin Beta B, inhibin beta B chain, Inhibin Subunit Beta B, inhibin, beta B, inhibin, beta B (activin AB beta polypeptide), Inhibin, beta-2

Entrez Gene IDs

3625 (Human); 16324 (Mouse); 25196 (Rat)

Gene Symbol

INHBB

Additional Activin B Products

Product Documents for Human Activin B Precursor Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human Activin B Precursor Antibody

For research use only

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