Mouse IgG NorthernLights™ NL557-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # NL007

NorthernLights 557 Fluorochrome-labeled Antibody, X Absorbed
R&D Systems, part of Bio-Techne

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry

Label

NL557 (Excitation = 557 nm, Emission = 574 nm)

Antibody Source

Polyclonal Donkey IgG

View all IgG Secondary Antibodies »

Product Specifications

Immunogen

Mouse IgG

Specificity

Mouse IgG (H+L)

Clonality

Polyclonal

Host

Donkey

Isotype

IgG

Scientific Data Images for Mouse IgG NorthernLights™ NL557-conjugated Antibody

Detection of Mouse IgG Primary Antibody by Immunocytochemistry.

Detection of human Vimentin by incubating cells with 8 μg/mL of Mouse anti-Human Vimentin monoclonal antibody (MAB21052) at room temperature for 1 hour followed by incubation with 1:200 dilution of Donkey Anti-Mouse IgG NorthernLights™ NL557-conjugated secondary antibody for 1 hour at room temperature. Note strong positive staining in A172 cells (left panel, positive control) and a lack of non-specific background staining in the absence of primary antibodies (right panel, negative control).

Applications for Mouse IgG NorthernLights™ NL557-conjugated Antibody

Application
Recommended Usage

Flow Cytometry

Use at a 1:200 dilution

Immunocytochemistry

Use at a 1:200 dilution

Immunohistochemistry

Use at a 1:200 dilution
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

*Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to the Safety Data Sheet (SDS) for additional information and handling instructions.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: IgG

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G

Alternate Names

Immunoglobulin G, ImmunoglobulinG

Additional IgG Products

Product Documents for Mouse IgG NorthernLights™ NL557-conjugated Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Mouse IgG NorthernLights™ NL557-conjugated Antibody

For research use only

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