Human Acylated Ghrelin Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8149

R&D Systems, part of Bio-Techne
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MAB8149
MAB8149-SP
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 891240

View all Ghrelin Antibodies »

Product Specifications

Immunogen

Human Ghrelin acylated peptide
Gly24-Arg51
Accession # Q9UBU3

Specificity

Detects only the acylated form of human Ghrelin in direct ELISA.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Acylated Ghrelin Antibody

Acylated Ghrelin antibody in Human Small Intestine Tissue by Immunohistochemistry (IHC-P).

Acylated Ghrelin in Human Small Intestine Tissue.

Acylated Ghrelin was detected in immersion fixed paraffin-embedded sections of human small intestine tissue using Mouse Anti-Human Acylated Ghrelin Monoclonal Antibody (Catalog # MAB8149) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to intestinal glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Acylated Ghrelin Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human small intestine tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Ghrelin

Mature Ghrelin peptides are the result of the clavage of the Ghrelin/Obestatin Prepropeptide, a 117 aa precursor peptide that is processed into three chains, Ghrelin-27 (aa 24-50), Ghrelin-28 (aa24-51) and Obestatin (aa76-98).  The prepropeptide is cleaved into proGhrelin, and then further processed into mature Ghrelin by prohormone convertases.  Mature Ghrelin is acylated with an N-octanoyl group on serine 3 which is required for receptor binding.  Ghrelin and Obestatin are predominantly synthesized in the gastric mucosa.  Ghrelin plays a role in growth factor release and appetite suppression as well as many other functions in a variety of organs.  Obestatin is a putative hormone that has been suggested to have opposite effects on growth factor release and appetite as Ghrelin.

Alternate Names

GHRL, Motilin-related Peptide, Protein M46

Entrez Gene IDs

51738 (Human); 58991 (Mouse); 59301 (Rat)

Gene Symbol

GHRL

UniProt

Q9UBU3 (Human)

Additional Ghrelin Products

Product Documents for Human Acylated Ghrelin Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Acylated Ghrelin Antibody

For research use only

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